Description

This track shows the exon junctions extracted from spliced RNA-Seq reads of multiple developmental stages of D. melanogaster. The RNA-Seq reads were obtained from the ENCODE data portal at UCSC on August 2019, and then mapped against the D. melanogaster dm6 genome assembly using HISAT2.

The splice junctions in each sample were identified by the regtools junctions extract subprogram. The splice junction predictions from the different samples are filtered and merged together into a single set of predictions. The predictions are color-coded based on the number of reads supporting the junction:

References

Brown JB, et al. Diversity and dynamics of the Drosophila transcriptome. Nature. 2014 Aug 28;512(7515):393-9.

Kim D, Langmead B, Salzberg SL. HISAT: a fast spliced aligner with low memory requirements. Nat Methods. 2015 Apr;12(4):357-60.